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| Product Name | Human Obestatin ELISA Assay Kit |
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| Description | This Human Obestatin ELISA Assay kit for determination of obestatin in human plasma or serum samples is based on a competitive enzyme immunoassay using the combination of highly specific antibody to human obestatin?and biotin-avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG, to which biotinylated human obestatin, human obestatin standard or samples and rabbit anti human obestatin antibody are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptavidin (SA) is added, so that HRP labeled SA-biotinylated human obestatin-antibody complex is formed on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3',5,5'-Tetramethylbenzidine (TMB) and the concentration of human obestatin is calculated. Sample Types: plasma, serum. Range: 0.231 - 25ng/mL. Time: 24 hours • Obestatin is a 23 amino acid residues peptide isolated from the rat stomach. The peptide shares the precursor with a food intake stimulating peptide, ghrelin, but possesses reducing effects on food intake, gut motility and body weight (1). With the use of an antiserum directed against the mouse/rat obestatin, obestatin immunoreactivity (irOBS) was detected in cells of the gastric mucosa and myenteric plexus and in Leydig cells of the testis in Sprague-Dawley rats. Double labeling of myenteric plexus with antisera against obestatin and choline acetyltransferase (ChAT) revealed that nearly all irOBS neurons were ChAT positive and vice versa (2). Obestatin (100nM) added to dissociated and cultured rat cerebral cortical neurons elevated cytosolic calcium concentrations [Ca+2]i in a population of cortical neurons (2). Intracerebroventricular admini- stration of obestatin inhibited water drinking in ad libitum fed and watered rats, and in food and water deprived animals. In addition, obestatin inhibited angiotensin II-induced water drinking in animals provided free access to water and food (3). Obestatin peptides had no effect on insulin sensitivity as revealed by hypoglycaemic response when co-administered with insulin, supporting a role of obestatin in regulating metabolism through changes of appetite, but indicating no direct actions on glucose homeostasis or insulin secretion (4). It is supposed that in rats the effects of obestatin on food intake may be secondary to an action of the peptide to inhibit water drinking (3). Recently, it is reported affording cardioprotection to ischemic- reperfused isolated rat heart, inhibiting apoptosis in culture of similarly stressed cardiomyocytes(5) and inhibiting dopamine release in rat hypothalamus(6). The obestatin concerning study for energy homeostasis and body weight regulation could be expected to have a large development in the future. The Eagle Biosciences Human Obestatin ELISA Assay kit developed by our laboratory can be used for direct determination of blood obestatin level's variations and will be a useful tool for further development of obestatin research. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Human Obestatin ELISA Assay Kit, YK231, Sceti |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | YK231 |
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| Order / More Info | Human Obestatin ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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