| 产品详情 |
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| Product Name | Anti tTg IgG (Tissue Transglutaminase) ELISA |
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| Description | The Eagle Biosciences Anti-hu tTG IgG ELISA Assay Kit is an enzyme immunoassay for the quantitative or semi-quantitative determination of IgG autoantibodies to tissue transglutaminase in human serum or plasma. Autoantibodies of the diluted patient samples, positive control, and calibrators react with human tissue transglutaminase immobilized on the solid phase of a microtiter plate. Anti-huTransG guarantees the specific binding of anti-tTG IgG autoantibodies of the specimen under investigation by employing highly purified, activated recombinant human tTG for coating. Following an incubation period of 60 min at room temperature (18...25°C), unbound serum components are removed by a wash step.The bound autoantibodies react specifically with anti-human-IgG-antibodies conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at room temperature. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step.HRP converts the colorless substrate solution of 3,3',5,5'-tetramethyl benzidine (TMB) added into a blue product. This enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 15 min at room temperature turning the solution from blue to yellow.The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the concentrations of the antibodies of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Sample Types: serum, plasma. Range: 1-300 U/mL. Sensitivity: 1U/mL. Time: 2.5 hours • Celiac disease, or gluten-sensitivity, is found already in neonates and is characterized by small intestinal damages leading to a so-called ``flat'' mucosa. Due to this extensive lesions mal-absorption occurs frequently accompanied with a depletion of key nutrients. Gliadin the alcohol soluble fraction of gluten represents the causative agent of celiac disease that provokes an inflammatory process in the small intestine. Gliadin is a substrate of tTG and cross-linked into high molecular complexes triggering probably both cellular and humoral immune responses. Incidence rates for celiac disease range from 1 in 300 (Western Ireland) to 1 in 4700 in European countries. However, a high number of subclinical cases of celiac disease have been detected by in-vitro tests revealing a prevalence of 4 in 1000. Individuals suffering from prolonged celiac disease additionally face an elevated risk of developing T cell lymphoma.Diagnosis of celiac disease comprises small intestine biopsy demonstrating a ``flat'' mucosa prior to a gluten-free diet and the following reconstitution of the mucosa after onset of the diet. Determination of anti-gliadin IgG and IgA by ELISA as well as the detection of anti-endomysium IgA by immunofluorescence has been considered as the main serological parameters for celiac disease so far.The identification of tissue transglutaminase as one of the main endomysial autoAntigenss and the availability of an easy to use and reliable ELISA kit employing recombinant human tissue transglutaminase pro mises the extension of diagnostic opportunities for celiac disease in future.IgA deficient celiac patients may demonstrate IgG autoantibodies to tTG only. Therefore, tTG IgG autoantibodies are the only serological parameter for celiac disease detectable in such patients apart from Gliadin IgG antibodies. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | TGG31-K01 ASSAY EIA ELISA IMMUNOASSAY KIT Anti tTG IgG Tissue transglutaminase, celiac |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | TGG31-K01 |
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| Order / More Info | Anti tTg IgG (Tissue Transglutaminase) ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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