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| Product Name | Human PMN Elastase ELISA Kit |
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| Description | The Eagle Biosciences PMN Elastase ELISA Assy employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for PMN Elastase has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any PMN Elastase present is bound by the immobilized antibody. After washing away any unbound substances, a HRP-conjugated antibody specific for PMN Elastase is added to each well and incubate. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of PMN Elastase bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450 nm ±2 nm.The concentration of PMN Elastase in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: plasma, seminal plasma, exudate, bronchoalveolar lavage fluid and cerebrospinal fluid. Range: 15.6 - 1000 ng/ml. Sensitivity: 0.2 ng/ml. Time: 2.25 hours • The human organism reacts with an inflammatory response to attacks of invading pathogens (micro-organisms and viruses) or damaged tissue (after accidents or surgery). Polymorphonuclear (PMN) granulocytes play an important role as primary defense cells in this inflammatory reaction. Different bloodstream mediators (cytokines, leukotrienes, complement factors, bacterial endotoxins, clotting and fibrinolysis factors) attract and stimulate these cells to phagocytize and destroy not naturally occurring agents. PMN granulocytes use proteinases to digest these agents and tissue debris. One of these proteinases is PMN elastase which is localized in the azurophilic granules of the polymorphonuclear granulocytes. During phagocytosis of foreign substances these enzymes are also partially excreted into the extracellular surrounding, where the activity of PMN elastase is regulated by inhibitors (esp. the α1-proteinase inhibitor, α1-PI). An overwhelming release of PMN elastase, however, can exceed the inhibitory potential of the α1-proteinase inhibitor. Thus, enzymatically active PMN elastase, together with simultaneously produced oxidants (O2-radicals, H2O2, OH-radicals), can cause local tissue injury. Due to the bloodstream and lymphatic system, however, α1-PI is delivered subsequently and eventually able to form a complex with all excreted elastase. Therefore, the concentration of the PMN elastase/ α1-PI complex correlates with the released PMN elastase and can be used as a measure for the activity of granulocytes during an inflammatory response. Primarily, determinations of PMN elastase find its application in observation of the course of trauma, shock and sepsis. Further indications are the areas of hemodialysis, infections by obstetrics, joint diseases, and effusions of sport injuries, intestinal affection, pancreatitis, cystic fibrosis and male adnex affections. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | PMN Elastase |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG80507 |
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| Order / More Info | Human PMN Elastase ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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