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| Product Name | Human PYY ELISA Assay Kit |
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| Description | This Eagle Biosciences ELISA Assay kit is for the determination of human PYY in samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to human PYY and biotin-avidin affinity system. To the wells of plate coated with rabbit anti human PYY antibody, standard or samples, labeled Antigens are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled streptoavidin-biotinylated Antigens-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3',5,5'-Tetramethyl- benzidine (TMB) and the concentration of human PYY is calculated. Sample Types: serum, plasma. Range: 0.082 - 20 ng/mL. Time: 24 hours • This Eagle Biosciences Human PYY ELISA is an enzyme immunoassay (EIA) kit and is a stable and convenient assay system for human peptide YY (PYY). PYY was isolated initially by Tatemoto et al. (1980) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acids residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in Endocrinology cells in the intestine (ileum, colon, and rectum). PYY shows an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level in human blood decreases after resection of the intestine, possibly be due to the decrease in number of the Endocrinology cells secreting PYY. The Human PYY ELISA Assay kit is prepared by using synthetic human PYY (3-36) as standard and biotinylated human PYY (3-36) as labeled Antigens. The kit can be used for measurement of PYY [both PYY (3-36) and PYY (1-36)] in human serum or plasma with high sensitivity. It will be a specifically useful tool for PYY research. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Human PYY ELISA Assay Kit |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | YK080 |
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| Order / More Info | Human PYY ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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