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| Product Name | Human Trail Receptor 1/DR4/CD261 ELISA Assay Kit |
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| Description | A capture Antibody highly specific for the Eagle Biosciences TRAIL R1 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of TRAIL R1 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed. During the next incubation period the binding of the biotinylated anti- TRAIL R1 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of TRAIL R1 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of TRAIL R1 in any sample tested. Sample Types: serum, cell lysates, cell culture supernatant. Range: 31.25-1000 pg/ml. Sensitivity: 8 pg/ml. Time: 2h30 • The Eagle Biosciences Human TRAIL R1/DR4 ELISA is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of TRAIL R1/DR4 in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human TRAIL R1/DR4. The Eagle Biosciences Human TRAIL R1/DR4 ELISA Assay kit is for Research Use Only. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Trail Receptor 1/DR4/CD261 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | C6131-K01 |
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| Order / More Info | Human Trail Receptor 1/DR4/CD261 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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