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| Product Name | Human VDBP (GC Globulin) ELISA Assay Kit |
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| Description | The Eagle Biosciences Human VDBP (GC Globulin) quantitative VDBP ELISA Assay Kit is a solid phase competitive immunoassay designed to detect VDBP. Microwells are coated with anti-VDBP antibody. Assay calibrators, control and diluted unknown serum or plasma specimens are added to the microwells along with GC Globulin HRP conjugated antibody. After an incubation period, the immunocomplex of solid phase bound ``VDBP Antibody-VDBP'' is formed, which inhibits the formation of ``VDBP Antibody - HRP Conjugated VDBP''. Unbound HRP Conjugated VDBP are removed with a washing step. During a second incubation with TMB substrate, a blue color is developed. The enzyme-substrate reaction is stopped by the addition of sulfuric acid. The absorbance of assay calibrators, controls and unknown specimens are measured by an ELISA plate reader with wavelength set at 450 nm. The color intensity is inversely proportional to the amount of VDBP present in the calibrators controls and specimens. Sample Types: serum, plasma. Range: 0 - 20.1 µg/mL. Sensitivity: 0.0637 µg/mL. Time: 1.5 hours • Vitamin D-binding protein (DBP) is a 58 kDa circulating glycoprotein secreted by the liver. It binds about 85% to 90% of circulating 25-OH-D2/3 and 1,25-(OH)2-D3, regulates the bioavailability of active vitamin D, and transports them to target organs. There is only less than one percent 25-OH-D2/3 is in the free form in the circulation. The full length DBP contains 476-amino acids, including a 16-amino acid signal sequence. Biologically, DBP may involve directly and indirectly in regulating bone metabolism. Circulating DBP also binds to actin at 1:1 molecule ratio, while this protein complex is removed by kidney. In patient with trauma, sepsis or multiple organ failure, DBP concentration decreases. Urine DBP is reported to be a biomarker of major renal event in patient undergoing coronary angiography. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | VDBP Vitamin D Binding Protein |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | DBP31-K01 |
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| Order / More Info | Human VDBP (GC Globulin) ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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