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| Product Name | Trastuzumab (Herceptin®) ELISA Assay Kit |
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| Description | This Eagle Biosciences ELISA Assay Kit is based on Trastuzumab-specific mouse monoclonal antibody (catcher Ab, ImmunoGuide clone VME-5A6b). Diluted standards and samples are incubated in the microtiter plate coated with IG-VME-5A6b mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Trastuzumab. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Trastuzumab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Trastuzumab to the solid phase, pre-coated with VME-5A6b, is inhibited by recombinant human HER2 protein in a concentration dependent manner. Therefore, the ImmunoGuide Trastuzumab ELISA (mAb-Based) measures the free form of Trastuzumab. Sample Types: serum, plasma. Range: 6 - 200 ng/mL. Sensitivity: 2 ng/mL. Time: 2 hours • The drug Trastuzumab (trade names Herceptin® or Herclon®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). The antibody is an IgG1 kappa that contains human framework regions with the complementarity-determining regions of a murine anti-p185 HER2 antibody that binds to HER2. Trastuzumab blood concentrations throughout the dosing interval expected to be remaining above those considered necessary for anticancer activity. Furthermore, in a separate analysis, patients with the lowest Trastuzumab serum trough concentrations had the highest rate of disease progression and shortest overall survival. This Eagle Biosciences ImmunoGuide Trastuzumab ELISA (mAb-based) is developed for the specific measurement of Trastuzumabin serum, plasma,and other biological fluids by the advantage of using a site-directed VME-5A6b mouse monoclonal antibody (mAb) specific for Trastuzumab only. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | anti-drug antibody kits, anti drug humira shikari shakari free ELISA Assay Kit EIA, Immunoassay, Trastuzumab, Herclon, Herceptin, IG-AB105, |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | IG-AB105 |
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| Order / More Info | Trastuzumab (Herceptin®) ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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