| 产品详情 |
| Edit |   |
|---|
| Product Name | Total Soluble Neuropilin-1 ELISA Assay Kit |
|---|
| Description | This kit is a sandwich enzyme immunoassay for the direct determination of total soluble Neuropilin-1 in humanserum and plasma samples. STD/CTRL/Sample require pre-treatment with an equal amount of guanidine hydrochloride (GuHCl) to remove potentially bound ligands, followed by pre-dilution with assay buffer. In a next step, pre-treated and diluted STD/CTRL/Sample and detection antibody (mouse monoclonal anti human Neuropilin-1 IgG) are pipetted into the wells of the microtiter strips, which are pre-coated with anti Neuropilin-1 antibody. Neuropilin-1 present in STD/CTRL/Sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. In the washing step all non-specific unbound material is removed. Subsequently, the conjugate (Streptavidin-HRPO) is pipetted into the wells and reacts with the detection antibody. After another washing step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed colour change of the substrate is directly proportional to the amount of Neuropilin-1. This colour change is detectable with a standard microtiter plate ELISA reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standard. The concentration of Neuropilin-1 in the sample is determined directly from the dose response curve. Sample Types: serum or plasma (citrate, EDTA or heparin). Range: 0.37 - 12 nmol/L. Sensitivity: LOD: (0 pmol/l + 3 SD): 0.09 pmol/l; LLOQ: 0.09 pmol/l. Time: 4 hours • Neuropilin-1 (NRP1) is a single-pass transmembrane glycoprotein of 923 amino acids, composed of a large extracellular region, a short transmembrane domain and a short cytoplasmic tail. Due to alternative splicing or shedding, the extracellular region can be released into circulation as a soluble Neuropilin. NRP1 is an essential cell surface receptor functioning in many key biological processes including the cardiovascular, neuronal, and immune systems. Multiple ligands binf to the extracellular region of NPR1, like class III semaphorins which have a key role in axonal guidance, or members of the VEGF family of angiogenic cytokines. Ligand-binding to transmembrane NRP1, which has co-receptor function, leads to signaling via receptor proteins containing a PDZ domain. In contrast, ligand-binding to soluble Neuropilin-1 (sNRP1) has antagonistic properties by acting as decoy. NRP1 is expressed by a variety of cells and tissues. For instance, the transmembrane protein is expressed by neuronal cells, endothelial cells, vascular smooth muscle cells, cardiomyocytes, multiple tumor cell lines and neoplasms, osteoblasts, naive T cells or platelets. Expression of soluble Neuropilin-1 is further described in a variety of non-endothelial cells, e.g. in liver hepatocytes and kidney distal and proximal tubules. NRP1 is implicated in a multitude of physiological and pathological settings, e.g. in axon guidance, vascularization, tumor growth or regeneration and repair. Neuropilin-1 is described to stimulate osteoblast differentiation, to act as potential biomarker for the prediction of heart failure outcome or to play a role in renal fibrogenesis. As a co-receptor for VEGF, NRP1 is a potential target for cancer therapies. |
|---|
| Size | 1 x 96 well |
|---|
| Concentration | n/a |
|---|
| Applications | RUO |
|---|
| Other Names | Neuropilin-1, |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | BI-20409 |
|---|
| Price | |
|---|
| Order / More Info | Total Soluble Neuropilin-1 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|