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| Product Name | Aldo-Keto reductase family1, member B1, 1-316aa, Recombinant, Human |
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| Description | Purity ≥ 95% (SDS-PAGE) Endotoxin: ≤1.0 EU/1ug of protein (LAL). Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Source: Human Aldose reductase (AKR1B1) protein was expressed in E. coli and purified by using conventional chromatography techniques. Specific Activity: ~0.2-0.9 units/mg protein. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Activity Assay: 1. Prepare a 750ul reaction mix into a suitable container. The final concentrations of the reaction mix components should be: 0.1M sodium phosphate (pH7.0) and 0.3mM NADPH. 2. Add 50ul of recombinant AKR1B1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 |
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| Size | 100ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | AKR1B1, ADR, ALDR1, ALR2, AR |
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| Gene, Accession, CAS # | Accession: NP_001619 |
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| Catalog # | A1334-70A |
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| Order / More Info | Aldo-Keto reductase family1, member B1, 1-316aa, Recombinant, Human from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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