| 产品详情 |
| Edit |   |
|---|
| Product Name | HAT Medium Supplement (Hypoxanthine-Aminopterin-Thymidine), 50X (Powder) |
|---|
| Description | The production of monoclonal antibodies involves the fusion of myeloma cells with spleen cells and the selection of hybridomas (cell hybrids) in culture utilizing hypoxanthine-aminopterin-thymidine (HAT) supplemented medium. Aminopterin blocks the synthesis of DNA by inhibiting dihydrofolate reductase Cells that lack the ability to utilize the salvage pathway for nucleotide synthesis are eliminated. Cells that possess hypoxanthine-guanine phosphoribosyl transferase (HPRTase) and thymidine kinase (TK) enzymes can utilize the salvage pathway if supplied with hypoxanthine and thymidine. The purpose of the medium is to: (1) selectively kill unfused myeloma cells that are well adapted to tissue culture and would otherwise outgrow any hybridomas produced and (2) eliminate any myeloma-myeloma hybridomas that lack HPRTase. HPRTase positive spleen-spleen hybridomas, although not sensitive to aminopterin, are normally short-lived in culture. After selection is complete (~10-14 weeks), aminopteri |
|---|
| Size | 10x10ml |
|---|
| Concentration | n/a |
|---|
| Applications | n/a |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | 216061 |
|---|
| Price | |
|---|
| Order / More Info | HAT Medium Supplement (Hypoxanthine-Aminopterin-Thymidine), 50X (Powder) from UNITED STATES BIOLOGICAL |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|