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| Product Name | Thioredoxin Reductase Activity, BioAssay™ Kit, Colorimetric (TrxR) |
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| Description | Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Sample Type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc. Specificity: Recognizes mammalian TrxR. Test Principle: The Thioredoxin Reductase BioAssay™ Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5'-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (lmax = 412nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specif |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 220324 |
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| Order / More Info | Thioredoxin Reductase Activity, BioAssay™ Kit, Colorimetric (TrxR) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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