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| Product Name | Tryptase, beta1, Recombinant, Mouse (TPSB1, MCPT7, Mast Cell Protease 7) |
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| Description | Source: Human CD33 Signal Peptide (Met 1- Ala 16) Mouse Tryptase beta-1 (Ile 29-Phe 273) Recombinant mouse Tyrptase beta-1/Mast Cell Protease 7 (rmTryptase beta-1, residues 29-273)1 in the above construct was expressed in a murine myeloma cell line, NS0. Molecular Mass: The purified, secreted recombinant, murineTryptase beta-1 corresponds to the mature form with the N-terminal sequence of I29VGGQEAHGN. By SDS-PAGE, the protein migrates as the doublet with apparent molecular masses of approximately 37 and 36kD. Purity: ≥ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤ 1EU/1ug of the protein as determined by the LAL method. Activity: Measured by its ability to cleave a peptide substrate, Benzyloxcarbonyl-Lys-Thiobenzyl ester (Z-K-SBzl), in the presence of 5,5'-Dithio-bis (1-nitrobenzoic acid) (DTNB), as measured by absorbance at 405nm and using the extinction coefficient of 13,260 M-1cm-1.2. The specific activity, measured with 100uM Z-K-SBzl and 500ng enzyme |
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| Size | 20ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | SwissProt: Q02844 |
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| Catalog # | T8700-02 |
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| Order / More Info | Tryptase, beta1, Recombinant, Mouse (TPSB1, MCPT7, Mast Cell Protease 7) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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