| 产品详情 |
| Edit |   |
|---|
| Antigenic Specificity | ZAP70 Phospho (Tyr292) |
|---|
| Clone | A16038A |
|---|
| Host Species | Mouse |
|---|
| Reactive Species | human, mouse |
|---|
| Isotype | IgG2b, κ |
|---|
| Format | purified |
|---|
| Size | 100 µg |
|---|
| Concentration | n/a |
|---|
| Applications | Western Blot (WB), Intracellular Staining for Flow Cytometry (ICFC) |
|---|
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
|---|
| Description | Purified anti-ZAP70 Phospho (Tyr292). ZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinase that is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and the related spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn.ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and by phosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr-292 has a negative regulatory role.Mutation of Y292 to phenylalanine enhanced NFAT induction in response to BCR stimulation, indicating phosphorylation of Y292 may promote association of ZAP-70 with a negative regulatory protein. ZAP-70 has been reported to bind Cbl following TCR stimulation and phosphorylation of Y292 was required for this interaction, as well as for the negative effect of Cbl on signaling. |
|---|
| Immunogen | Human ZAP70 peptide phosphorylated at Y292. |
|---|
| Other Names | p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70 |
|---|
| Gene, Accession # | Gene ID: 7535 |
|---|
| Catalog # | 691902 |
|---|
| Price | |
|---|
| Order / More Info | ZAP70 Phospho (Tyr292) Antibody from BIOLEGEND |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|