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Product Name | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 488 |
Description | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 488, eBioscience™. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immuno |
Size | 25 µg |
Concentration | n/a |
Applications | Control: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: Assay-Dependent, Immunofluorescence: Assay-Dependent, Immunohistochemistry: Assay-Dependent |
Other Names | IgG2; IgG2b; IgG2bk |
Gene, Accession, CAS # | n/a |
Catalog # | 53-4031-80 |
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Order / More Info | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 488 from INVITROGEN ANTIBODIES |
Product Specific References | PubMed: 24973296 |
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