| 产品详情 |
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| Antigenic Specificity | SMAD3 phospho S423/phospho S425, internal |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine, chicken |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 25 µL |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known. |
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| Immunogen | Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein. |
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| Other Names | rabbit anti-SMAD3 pS423pS425 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2 |
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| Gene, Accession # | SMAD3, NCBI: 5174513, UniProt: P84022 |
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| Catalog # | 600-401-919S |
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| Price | |
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| Order / More Info | SMAD3 phospho S423/phospho S425, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Yamashita M, Fatyol K, Jin C, Wang X, Liu Z and Zhang YE. (2008) TRAF6 Mediates Smad-Independent Activation of JNK and p38 by TGFss. Molecular Cell 31, 918-924.Herhaus L, Al-Salihi M, Macartney T, Weidlich S, Sapkota GP. (2013) OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3. Nat Commun. 2013;4:2519. doi: 10.1038/ncomms3519.Tripathi V, Sixt KM, Gao S, Xu X, Huang J, Weigert R, Zhou M, Zhang YE. (2016) Direct Regulation of Alternative Splicing by SMAD3 through PCBP1 Is Essential to the Tumor-Promoting Role of TGF-?. Mol Cell. 2016 Dec 1;64(5):1010. doi: 10.1016/j.molcel.2016.11.025. |
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