| 产品详情 |
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| Antigenic Specificity | SMAD3 phospho T179, internal |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, mouse |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 100 µg |
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| Concentration | 1.0mg/ml |
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| Applications | ELISA, Western Blot. Anti-SMAD3 pT179 has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-SMAD3 pT179 affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known. |
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| Immunogen | Anti-SMAD3 pT179 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179. |
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| Other Names | rabbit anti-SMAD3 pT179 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2 |
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| Gene, Accession # | SMAD3, Gene ID: 4088, NCBI: NP_005893, UniProt: P84022 |
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| Catalog # | 600-401-C48 |
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| Price | |
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| Order / More Info | SMAD3 phospho T179, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Herhaus L, Al-Salihi M, Macartney T, Weidlich S, Sapkota GP. (2013) OTUB1 enhances TGF? signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3. Nat Commun. 2013;4:2519. doi: 10.1038/ncomms3519.Tripathi V, Sixt KM, Gao S, Xu X, Huang J, Weigert R, Zhou M, Zhang YE. (2016) Direct Regulation of Alternative Splicing by SMAD3 through PCBP1 Is Essential to the Tumor-Promoting Role of TGF-?. Mol Cell. 2016 Dec 1;64(5):1010. doi: 10.1016/j.molcel.2016.11.025. |
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