| 产品详情 |
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| Antigenic Specificity | CD3 epsilon |
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| Clone | APA1/1 |
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| Host Species | Mouse |
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| Reactive Species | human, mouse |
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| Isotype | IgG1 |
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| Format | Protein A purified |
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| Size | 0.1 mg |
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| Concentration | 1.0 mg/ml |
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| Applications | Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, CyTOF-ready. Flow Cytometry: Sample preparation - At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 ug/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. Immunocytochemistry: Fixed and permeabilized cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. This antibody is CyTOF ready. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The CD3 epsilon Antibody (APA1/1) from Novus Biologicals is a mouse monoclonal antibody to CD3 epsilon. This antibody reacts with human, mouse. The CD3 epsilon Antibody (APA1/1) has been validated for the following applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, CyTOF-ready. The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon |
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| Immunogen | Purified human CD3 proteins isolated from thymus |
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| Other Names | CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, FLJ18683, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE |
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| Gene, Accession # | CD3E, Gene ID: 916 |
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| Catalog # | NBP1-30426 |
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| Price | |
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| Order / More Info | CD3 epsilon Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | n/a |
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| 产品资料 |
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