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| Product Name | EZ4U Cell Proliferation Assay |
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| Description | The assay set-up is performed in a manner similar to the standard 3H-thymidine incorporation method. Instead of pulsing with tritiated nucleotide, 20 µl of dye solution is added to 200 µl sample. Incubation time is dependent on the metabolic capacity of the cells. Usually 2 to 5 hours of incubation at 37°C are sufficient to yield a significant increase in color intensity. As different cells vary in their ability to convert the yellow colored tetrazolium compound to its red formazan derivative, we recommend testing every new cell-line's metabolic capacity as described in Fig.2. After incubation, the plate is removed from the incubator and gently mixed by tipping the plate at all four sides. To avoid increased standard deviations, the plate must be shaken before reading the optical density. • Proliferation assays are widely used in cell biology for the study of growth factors, cytokines, nutrients and for the screening of cytotoxic or chemotherapeutic agents. There are several ways to determine the number of cells either by microscopic inspection, or by the use of an electronic particle counter, indirectly by measuring the incorporation of radioactive precursors, quantitating total protein with chromogenic dyes, or by measuring metabolic activity of cellular enzymes. The most common assay for cell proliferation is the incorporation of 3H?thymidine into cellular DNA. The 3H-thymidine assay is, however, labour intensive as it requires the removal of excess, unincorporated label by using some method of cell harvesting before measurement. In 1956, the first paper was published on the use of tetrazolium salts as indicators of cell viability. The method was based on the finding that living cells are capable to reduce slightly or uncoloured tetrazolium salts into intensely coloured formazan derivatives. This reduction process requires functional mitochondria, which are inactivated within a few minutes after cell death. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | BI-5000 |
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| Order / More Info | EZ4U Cell Proliferation Assay from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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