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| Antigenic Specificity | Cytokeratin, pan |
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| Clone | AE1 + AE3 |
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| Host Species | Mouse |
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| Reactive Species | human, mouse, rat, bovine, canine, chicken, primate, rabbit, reptile |
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| Isotype | IgG1 kappa |
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| Format | Protein A/G purified |
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| Size | 20 ug |
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| Concentration | 0.2 mg/ml |
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| Applications | Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Single Cell Western. Mol. Weight of Antigen: 40 - 67 kDa 1. The staining pattern of the pan cytokeratin antibody cocktail may be different than that of either antibody separately. 2. The pan cytokeratin cocktail does not react with keratin18, which is also expressed in carcinomas. As such, negative staining with NBP2-29429 in of itself may not be sufficient evidence to rule out the possibility of a carcinoma (Ordonez, 2013). a. For example, hepatocellular, adrenal cortical, clear cell renal and chromophobe renal cell carcinomas have been reported to be negative for the pan cytokeratin antibody. In this regard, the pan cytokeratin antibody can be used as part of a screening panel to more extensively define the tumor cell lineages. 3. The pan cytokeratin antibody may cross-react with GFAP, leading to aberrant positive staining of glial tumors such as ependymoma, glioblastoma, or schwannoma (Ordonez, 2013). |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The Cytokeratin, pan Antibody (AE1 + AE3) from Novus Biologicals is a mouse monoclonal antibody to Cytokeratin, pan. This antibody reacts with human, mouse, rat, bovine, canine, chicken, primate, rabbit, reptile. The Cytokeratin, pan Antibody (AE1 + AE3) has been validated for the following applications: Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), Single Cell Western. |
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| Immunogen | Total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982). |
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| Other Names | n/a |
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| Gene, Accession # | KRT1 |
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| Catalog # | NBP2-29429-20ug |
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| Price | |
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| Order / More Info | Cytokeratin, pan Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 11351328, 12411988, 15304163, 15328170, 17131867, 17230512, 17442382, 23427873, 29169625, 29627937, 6183275 |
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