| 产品详情 |
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| Product Name | N-MID® Osteocalcin ELISA |
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| Description | The N-MID® Osteocalcin ELISA is based upon the application of two highly specific monoclonal antibodies (Mabs) against human osteocalcin. An antibody recognizing the midregion (amino acids 20-29) is used as the capture antibody and for detection a peroxidase conjugated antibody recognizing the N-terminal region (amino acids 10-16) is used. In addition to intact osteocalcin (amino acid 1-49) the N-terminal-Mid fragment (amino acids 1-43) is also detected. Standards, control and unknown samples are pipetted into the appropriate microtitre wells coated with streptavidin. Then a mixture of a biotinylated antibody and a peroxidase conjugated antibody is added. Following incubation for 2 hours at room temperature the wells are washed and a chromogenic substrate is added and the colour reaction is stopped with sulfuric acid. Finally, the absorbance is measured. |
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| Size | 96 wells |
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| Concentration | n/a |
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| Applications | colorimetric ELISA or EIA |
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| Other Names | osteocalcin kit, assay, immunoassay |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | AC-11F1 |
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| Order / More Info | N-MID® Osteocalcin ELISA from IMMUNODIAGNOSTIC SYSTEMS |
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| Product Specific References | n/a |
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