| 产品详情 |
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| Product Name | FSH ELISA |
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| Description | In the FSH ELISA Assay Kit ,the essential reagents required for an immunoenzymatic assay include high affinity and specificity antibodies (enzyme-linked and immobilised) with different and distinct epitope recognition, in excess, and native Antigens. In this procedure the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti FSH antibody. Upon mixing monoclonal biotinylated antibody, the enzyme labeled antibody and a serum containing the native Antigens, reation results between the native Antigens and the antibodies without competition or steric hindrance to form a soluble sandwich complex. The interaction is illustrated by the following equation: Ka EnzAb(p) + AgFSH + BtnAb(m) ? EnzAb(p)- AgFSH- BtnAb(m) K-a BtnAb(m) = biotinylated monoclonal antibody (Excess quantity) AgFSH = native FSH Antigens (variable quantity) EnzAb(p) = enzyme labeled policlonal antibody (Excess quantity) EnzAb(p)- AgFSH- BtnAb(m) = Antigens-antibodies sandwich complex Ka = rate constant of association K-a = rate constant of disassociation Simultaneously the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody. This interaction is illustrated below: EnzAb(p)-AgFSH-BtnAb(m) + streptavidincw ? immobilized complex Streptavidincw = streptavidin immobilized on well Immobilized complex = antibodies-Antigens sandwich bound. After equilibrium is attained, the antibody-bound fraction is separated from unbound Antigens by a washing step. The enzyme activity in the antibody-bound fraction is directly proportional to the native Antigens concentration. By using several different serum references of known Antigens values, a dose response curve can be generated from which the Antigens concentration of an unknown can be ascertained. Sample Types: serum, plasma. Range: 5 -100 mIU/ml. Sensitivity: 0.17 mIU/ml. Time: 1.5 hours • Follicle Stimulating hormone (FSH) is a glycoprotein consisting of two subunits with an approximate molecular mass of 35,500 daltons. The α- subunit is similar to other pituitary hormones [luteinizing stimulating hormone (LH), thyroid stimulating hormone (TSH) and chorionic gonadotropin (hCG)] while the β-subunit is unique. The β- subunit confers the biological activity to the molecule. Stimulation by gonadotropin-releasing hormone (GnRH) causes release of FSH, as well as LH, from the pituitary and is transported by the blood to their sites of action, the testes or ovary. In men, FSH acts on the Sertoli cells of the testis, stimulating the synthesis of inhibin, which appears to specifically inhibit further FSH secretion, and androgen-binding protein. Thus, it indirectly supports spermatogenesis. In women, FSH acts on the granulosa cells of the ovary, stimulatin steroidogensis. All ovulatory menstrual cycles have a characteristic pattern of FSH, as well as LH, secretion. The menstrual cycle is divided into a follicular phase and a luteal phase by the midcycle surge of the gonadotropins (LH and FSH). As the follicular phase progresses, FSH concentration decreases. Near the time ovulation occur, about midcycle, FSH peaks (lesser in magnitude than LH) to its highest level. The clinical usefulness of the measurement of Follicle Stimulating hormone (FSH) in ascertaining the homeostasis of fertility regulation via the hypothalamic - pituitary - gonadal axis has been well established (1,2). |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Follicle Stimulating hormone (FSH), steroid hormone, fertility regulation |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | DCM010 |
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| Order / More Info | FSH ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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