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| Product Name | Fecal Giardia lamblia Antigens ELISA Assay Kit Assay Kit |
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| Description | This Eagle Biosciences ``sandwich'' Fecal Giardia Iamblia Antigens ELISA is designed, developed and produced for the qualitative measurement of Giardia lamblia Antigens in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter well. Assay controls and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified polyclonal anti-Giardia lamblia antibody on its wall. The Giardia lamblia Antigens will be bound to the antibody coated plate after an incubation period. The unbound matrices are washed away and a HRP-conjugated monoclonal antibody which specifically recognizes the protein of Giardia lamblia is added for further immunoreactions. After an incubation period, an immunocomplex of ``Anti-Giardia Antibody - Giardia lamblia Antigens - HRP-conjugated Anti-Giardia Tracer Antibody'' is formed if Giardia lamblia Antigens is present in the test sample. The unbound tracer antibody and other protein or buffer matrix are removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to giardia proteins captured on the wall of each microtiter well is directly proportional to the amount of Giardia lamblia Antigens level in each test specimen. Sample Types: stool. Range: Cut-off. Sensitivity: Cut-off. Time: 2 hours • Giardia lamblia (also known as Giardia intestinalis) has a characteristic tear-drop shape and measures 10-15 µm in length. It has twin nuclei and an adhesive disk which is a rigid structure reinforced by supelicular microtubules. There are two median bodies of unknown function, but their shape is important for differentiating between species. There are 4 pairs of flagella, one anterior pair, two posterior pairs and a caudal pair. These organisms have no mitochondria, endoplasmic reticulum, golgi, or lysosomes. Giardia has a two-stage life cycle consisting of trophozoite and cyst. The life cycle begins with ingested cysts, which release trophozoites (10-20 µm x 5-15 µm) in the duodenum. These trophozoites attach to the surface of the intestinal epithelium using a ventral sucking disk and then reproduce by binary fission. The trigger for encystment is unclear, but the process results in the inactive, environmentally resistant form of Giardia -- a cyst (11-14 µm x 7-10 µm) that is excreted in feces. Giardiasis is a diarrheal illness caused by Giardia lamblia, after ingestion of Giardia cysts. Once a person has been infected with Giardia, the parasite lives in the intestine and is passed in the stool. Millions of germs can be released in a bowel movement from an infected human or animal. Giardia is found in soil, food, water, or surfaces that have been contaminated with the feces from infected humans or animals. Because the parasite is protected by an outer shell, it can survive outside the body and in the environment for long periods of time. Because it is spread world-wide, Giardia lamblia has become one of the most important causes of chronic diarrhea. About 15-20% of children under age ten years and 19% of male homosexuals have been infected. Giardia infection can cause a variety of intestinal symptoms either acute or chronic, which include diarrhea, gas or flatulence, greasy stools that tend to float, stomach cramps, upset stomach or nausea. These symptoms may lead to weight loss and dehydration. Some people with giardiasis have no symptoms at all. Those asymptomatic cases still shed Giardia cysts. Generally, symptoms of giardiasis begin 1 to 2 weeks after becoming infected and they may last 2 to 6 weeks. The method used for the diagnosis of giardiasis in the past has been the detection of Giardia cysts in stool by microscopy. Recently, specific Giardia Antigens ELISA greatly simplified the diagnostic procedure and is as sensitive as the microscopic method. Another advantage of using Giardia Antigens ELISA is that it does not require the intact organisms in the test specimen. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Giardia Iamblia Antigens |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | GIA35-K01 |
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| Order / More Info | Fecal Giardia lamblia Antigens ELISA Assay Kit Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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