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| Product Name | Fecal/Tissue Cortisol ELISA |
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| Description | This assay employs the competitive enzyme immunoassay technique. A specific anti-mouse-IgG antibody has been pre-coated onto a microtiter plate. Cortisol containing samples or standards and a Cortisol -HRP conjugate are given into the wells of the microtiter plate. Then a mouse monoclonal antibody to Cortisol is added into the wells. Cortisol in samples or standards and a fixed amount of Cortisol -HRP conjugate are competing for a limited amount of Cortisol mouse monoclonal antibody. The Cortisol-antibody complex binds tothe goat polyclonal anti-mouse IgG that has been previously bound to the well. After incubation, the wells are washed with wash buffer to remove unbound material. A substrate solution is then added and incubated, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured at 450 nm. The concentration of Cortisol is indirectly proportional to the color intensity of the test sample. Sample Types: dried fecal samples, tissue culture media and urine. Range: 100 - 3200 pg/ml. Sensitivity: 17.3 pg/ml. Time: 1.5 hours • Cortisol is a steroid hormone, in the glucocorticoid class of hormones. When used as a medication, it is known as hydrocortisone. It is produced in humans by the zona fasciculata of the adrenal cortex within the adrenal gland. It is released in response to stress and low blood-glucose concentration. It functions to increase blood sugar through gluconeogenesis, to suppress the immune system, and to aid in the metabolism of fat, protein, and carbohydrates. It also decreases bone formation. Cortisol is often referred to as the ``stress hormone'' as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance. In animals, cortisol is often used as an indicator of stress and can be measured in blood, saliva, urine, hair, and faeces. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Cortisol, Steroid, hormone |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG81392 |
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| Order / More Info | Fecal/Tissue Cortisol ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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