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Enzymatic Chromatrap® Standard Pro-G spin column shearing kit
Enzymatic Chromatrap® Standard Pro-G spin column shearing kit
Brand:
Porvair Sciences Limited
Model:
Standard Pro-G
Updated:
2/16/2022
  
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  • Product Detail

    Product Description

    24 Chromatrap®  Protein-G based spin-columns, buffers and reagents for enzymatic shearing of chromatin, for ChIP assays.

    The kit contains sufficient Protein-A based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.

    Standard kits contain all of the buffers and filter materails, but do not contain the positive or negative control antibodies.

    Kit Contents:

    Chromatrap® spin columns 24
    Column conditioning buffer 50mls
    Wash Buffer 1  50mls
    Wash Buffer 2  50mls
    Wash Buffer 3 50mls
    1.3M Glycine  20mls
    Lysis Buffer 10mls
    Digestion Buffer 10mls
    Enzymatic Stop Solution 200µl
    0.1M Elution Solution 3mls
    0.1M NaOH  3mls
    5M NaCl 500µl
    1M NaHCO3 500µl
    Hypotonic Buffer  10mls
    Shearing Cocktail 100µl
    Protease Inhibitor Cocktail (PIC) 100µl
    Proteinase K stop solution 100µl
    Proteinase K  50µl
    1.5ml Collection tube 24

    Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.

    Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.

    Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.

    For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.salesporvair-sciences.com.
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