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- Product Detail
- True 3D micro-environment
- Minimal protocol adaption required to switch from 2D to 3D
- Compatible with industry-standard automated handling and imaging equipment
- High well-to-well and batch-to-batch consistency
- Scaffolds are free from animal-derived products and synthesised using medical-grade polymers.
- Compatible with fluorescent and light microscopy
- Supplied gamma-irradiated in individually-sealed plastic wrapping
- Scaffolds can be coated with materials to facilitate cell adhesion in low serum conditions
- Material: medical-grade poly-L-lactide (PLLA), FDA-approved
- Scaffold thickness: 50 µm
- Available fibre diameters: 4 µm (= pores of 15-30 µm)
- Overall porosity: app. 80%
- Non-biodegradable in in vitro applications
- Supplied with low profile lid with condensation rings
- Instructions for seeding cells
- Cell viability assays used
- Optimised cell lysis for RNA preparation
- Optimised cell recovery
- Instructions for imaging cells
- - Mimetix® Protocol: Cell Viability Assays
- Mimetix® Protocol: Cell Visualisation
- Mimetix® Protocol: Cell Recovery & Lysis
ApplicationMimetix® scaffold have proven to be versatile and consistent environment for various research fields. Here we highlight a few experimental outcomes achieved using these electrospun scaffolds:
Cell imaging
HepG2 were seeded at 10,000 cells per well and fixed. Nuclei were stained with DAPI, actin filaments with Alexa fluor 488 and imaging was performed using a Nikon Eclipse C1 confocal microscope half way down the scaffold (objective x60). Unlike in the 2D environment, liver cancer cells establish a 3D network in the Mimetix scaffold
Primary dermal fibroblasts were cultured on our randomly orientated fibre scaffold and images were taken after 24 hours and 7 days. After 7 days in culture, cells had proliferated extensively to fill the pores within the scaffold.
High Throughput Myelination Assay
Our aligned electrospun Mimetix scaffold allows: - Differentiation of more than 80% of oligodendrocyte precursors into mature oligodendrocytes in 14 days
- Production of sheath lengths comparable to in vivo situation within 7 days by 90% of the oligodendrocytes
- Physical guidance and support for Schwann cells
- Metabolic functions are preserved in Mimetix® for up to 28 days
- CYP activities are similar to those in primary hepatocytes showcasing enzymatic drug metabolism in-vitro
- Phase II enzymes that are involved in solubility of drugs and hormones as well as excretable metabolites display same or increased expression in Mimetix® scaffolds compared to 2D controls
- The extensive design and manufacturing capabilities allow us to develop bespoke electrospun scaffolds from a large variety of polymers and in custom formats.
- Electrospun scaffolds can be designed to mimic the extracellular matrix in terms of its architecture, chemical functionality and mechanical properties, therefore providing an ideal substrate for cell growth, differentiation and ultimately tissue repair.
- Materials can be manufactured into implantable medical devices, coatings to improve the acceptance of implants, or supports for implantation of autologous or allogeneic cells for reconstructive therapy.
- Scaffolds can be made from FDA-approved polymers and formulated to degrade over a defined period in vivo or be used as carriers for therapeutic compounds.
- Bioactive compounds can be incorporated into the fibre structure for targeted release or modifying surface properties.
- We can blend or co-spin various polymers, and can form complex nano-composite materials.
Mimetix® 3D Cell Culture
Mimetix® scaffolds mimics the extracellular matrix by providing an ideal architectural environment to support the growth of cells in 3D. They are created by electrospinning the medical-grade polymer poly(L-lactide) (PLLA) into microfibers, which are highly consistent with regard to fibre diameter and pore size, resulting in excellent reproducibility of cell-based assays.
The Mimetix® scaffold is incorporated into standard SBS footprint well plate frames with bases of superior optical clarity and minimal base distortion. The scaffold depth of 50 µm is thick enough to provide the benefits of 3D cell morphology and behaviour, yet thin enough to allow microscopic imaging
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Advantages |
Figure 1. Co-culture of primary keratinocytes and primary dermal fibroblasts in the Mimetix®scaffold after 24 h (fibroblasts in the background, keratinocytes in the foreground). Courtesy of Dr A.J. Bullock, University of Sheffield
Usage and Handling
Scaffold Specifications:
| Workflow | 2D | Mimetix® scaffold |
| Preconditioning | Not necessary | Wet with 20% ethanol, 2 subsequent washing steps with PBS and leave in culture medium. |
| Coating | Possible | As easy as 2D. Larger amounts of the coating agent might be required. |
| Cell seeding | Varies | Depends on cell type. Using a similar cell number for seeding than in 2D is a good starting point, but ideally a range of different seeding densities should be tested. |
| Medium exchange | Easy | As easy as 2D |
| Plate reader analysis | Easy | Fluorescence and Luminescence-based assays: as easy as 2D. Absorbance-based assays: supernatant has to be transferred to a clear plate prior to read-out |
| Flow cytometry analysis | Easy | Same as 2D. Potentially longer incubation times/more rigorous shaking with lysis agent needed; please refer to protocol “Optimised cell recovery” for more information”. |
| Microscopic analysis | Easy | Confocal fluorescent microscopy: as easy as 2D. Confocal brightfield microscopy: as easy as 2D. Standard optical microscopy: does not work well because of scaffold opacity |
| Ease of Automation | Easy | As easy as 2D |
Product Manual
For more information download our Mimetix® product manual featuring:
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| Cortical oligodendrocyte precursors differentiating into active oligodendrocytes and lay myelin on the Mimetix scaffold in the absence of neurons. Cells stained after 14 days for myelin basic protein (green) and Hoechst (blue). | Percent of oligodendrocyte precursors (NG2) and oligodendrocytes (MBP). More than 600 cells were counted per condition. Mean and standard deviation are shown for three experiments. | Schwann cells grown for 21 days and stained with anti S-100 (purple) and Hoechst (blue). |
Download High Throughput Myelination Presentation
Mimetix Aligned scaffolds improve both structural and functional read-outs in cardiomyocytes, growing 3D cultures of spontaneously beating hiPSC-derived cardiomyocytes (hiPSC-CMs) in 96-well format. hiPSC-CMs grown on such aligned 3D plates showed statistically significantly higher Ca2+ transient rising slope (indicating faster kinetics), lower peak width durations, and lower amplitudes as compared to standard 2D tissue culture plates.
Download presentation (Experimental work performed at Merck, USA)
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| Differentiation of neural stem cells into mature neurons within the Mimetix® scaffold. Cells are stained with DCX (red) Tubulin βIII (green) DAPI (blue). | Neurite outgrowth on our Mimetix® scaffold. Cells are stained with DAPI (blue), Nestin (green) and GFAP (red). Both images: cell cultures on 12-well plate; courtesy of Lara Stevanato, PhD, ReNeuron. |
Electrospinning is an established method of producing nanofibres from a wide variety of natural and synthetic polymers. A polymer solution is injected at a constant feed rate though a nozzle or needle which is charged to a high voltage, typically 10 to 30 kV. (more...)
Mimetix® scaffolds are produced in Class VII cleanroom using state-of-the art electrospinning equipment from IME Technologies and are ISO 13485 certified. Each scaffold batch is checked under a scanning electron microscope (SEM) before shipment to the customer.
AMSBIO offers a custom service for electrospun fibres. These scaffolds can be precisely engineered based customer's choice of material, format, fibre diameter etc. Please find our request form for electrospun scaffolds to place an order for your very own scaffold.
The development process and its documentation comply with ISO 13485 Quality Management System.
Related Areas
Recombinant Animal Free Extracellular Matrix 3D Hydrogels
Cultrex Basement Membrane Extracts (BME)
2D Animal free, chemically defined extracellular matrices
Detachin Cell Detachment Solution
* AMSBIO is the global source for Mimetix®. Mimetix® is a registered trade mark of and manufactured by Electrospinning Company.
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AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.
Formed in 1987 by Sandy Allan and Alex Sim the company’s first offices were in Madrid, Spain. Over the following three years further offices were opened in Italy, Switzerland and the UK. During the formative years of existence AMS helped launch the successful sales campaigns of Stratagene and Invitrogen providing them with an important foothold from which to develop their global brand expansion.
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