1300A 系列原位/体内/离体肌肉测试系统

拉长收缩,疲劳,硬度,力度-收缩速率和功循环测试系统

特点:

1. 高效的三合一系统设计:

1.1) 原位实验

1.2) 活体动物体内实验(踏板设计)

1.3) 离体实验(浴盆)

2.适用于损伤和纵长等课题的活体动物实验

3.可选择25ml 浴盘应用于离体肌肉实验

4.操作简单的高产量测试系統， 适用于任何经验水平的研究者

概述：

1300A 三合一测试系统的设计主要是让肌肉生理学家能够通过简易的操作去测试出大鼠或小鼠等肌肉组织的机械te性和功能参数。

1300A拥有一个te的双模肌肉杠杆系统, 通过连接着肌肉其中一端肌腱的基础上， 同时进行肌肉在收缩力度和长度变化两方面的测量和控制，这大大地节省了实验需时和提高了实验的效能。

1300A测试系统可应用于拉长收缩,疲劳,硬度,力度-收缩速率和功循环等实验方案， 同时具有高弹性尺度， 可以应用於等距测试方案， 例如抽搐， 强直收缩和力量-刺激频率等实验方案。

三个不同的实验装置(体内/离体/原位)可以轻易地在该系统之中进行转换， 例如从体内实验装置(踏板设计)转换至离体实验装置(25ml浴盆)，过程只需要数分钟的时间。

系统包括:

1.肌肉杠杆系统:测试和控制力度和长度 (大力度值10N/1000grams)

2.可控温的精密动物平台

3 .双模 (电流和电压) 神经刺激器

4.桌用电脑 (附加萤幕) ﹕ 配置控制和分析软件系统

5.可附加 25ml 离体实验浴盆

特点:

1.设计应用于肌纤维， 肌肉束，单条肌肉或细小的肌肉如trabeculae

2.适用于长度-张力， 力-收缩速率和硬度等的测量

?3.力度峰值达至100mN

4.可控温度范围: 0-40°C

5.400μL 或1900μL循环灌流浴， 包括两条铂电ji用以提供刺激

6.可以配合标准显微镜或者倒置显微镜

7.可选择单一等距实验模式

概述：

 1500A是一个一站式系统， 可以让从事生理学研究方面 的科学家更简单快捷地去测试细小组织的机械te性，例如普遍的力度参量。

该系统的实验装置配合于绝大部份的倒置显微镜， 应用于成像观察和萤光测量。实验装置拥有两个不同大小的浴盘供以选择去对应细小或是中等尺寸的样本。可控温浴盆备有溶液循环灌流的装置和两条内置的铂金电ji以提供不同型态的电刺激。系统同时备有一个1L水库为浴盆供应含氧媒体。

1500A 可以测试控制的力度和长度， 使其应用范围更广泛， 从简单的张力测试去到样本组织完整的机械te性(例如长度-张力，力-收缩速率和硬度)都能够被测试。附加的软件程序(600A) 备有各种标的实验方案去配合不同的实验需求。

力传感器通过浴盆其中一端的小槽与样本组织连接着， 该设计令浴盆的底部可以被有机玻璃密封， 从而非常适用于进行人工心肌组织或一些高氧耗量的研究。因为配置限制性很低， 1500A 是一个非常里想的测试系统去应用于细小肌肉组织方面的实验。

完整的系統包括:

● 高速长度操控仪

●  400A力传感器

● 高配置度的实验浴盆

● 电场刺激器

●桌用电脑 (附加萤幕) ﹕ 配置控制和分析软件系统

● 温度控制器和冷却液循环器

● 1升灌流水库

● 可附加肌小节间隔长度检测软件(HVSL/ VSL)

六、1600A透性肌肉(去膜)细胞测试系统

型号:1600A

价格:请致电：010-67529703

品牌:美国aurora

1600A 透性肌肉细胞测试系统

特点:

?  应用于透性 (去膜) 心肌细胞的研究

?  测试kTR, 力-钙离子浓度, 长度-张力, 力-收缩速率和硬度等机械te性

?  备有8个溶液小槽的寛阔平台以盛载不同浓度的钙离子溶液

?  电动化三维微定位装置以准确和快速地将样本定位

?  可以配合标准显微镜或者倒置显微镜

?  可控温度范围: 0-40°C

1600A 是一个可靠和可重复性的实验测试系统，应用于测试去膜心肌细胞的主动和被动性质。透过使用其摇杆控制电动化定位装置可以轻易地令细胞的两端分别连接于力传感器和高速伺服电机，这大大提高了实验效能和简化了原本精细的样本连接程序。

可控温浴盆拥有8个溶液小槽令细胞可以轻易地在不同浓度的钙离子溶液之间进行转换，该装置会直接放置在倒置显微镜的置物平台上进行高清晰度的观察和肌节间距的检测。可选择附加一块棱镜去准确地检测出细胞于溶液槽的深度。

1400A测试系统可以控制和测试力度， 长度和肌小节的间隔长度(当使用可附加的高速视频肌节长度系统) ，使其应用范围更广泛， 从简单的力-钙离子浓度曲线去到样本完整的机械te性(例如kTR， 长度-张力， 力-收缩速率和硬度) 都能够被测试。附加的软件程序(600A) 备有各种标准的实验方案去配合不同的实验需求。

完整系统包括:

●高速长度操控仪

● 400A力传感器

●电动化三维微定位平台

●带有8个溶液小槽的可控温平台

●客制桌用电脑(附加萤幕): 配有控制和分析软件系统

●温度控制器和冷却液循环器

●可附加肌小节间隔长度检测软件(HVSL/ VSL)

应用文献：

一、心肌细胞和肌肉文献

American Journal of …, 2005 - Am Physiological Soc

... Power output is linearly related to MyHC content in rat skinned myocytes and isolated working

hearts. ... were made using a capacitance-gauge transducer (model 403; sensitivity, 20 mV/mg plus

a x10 amplifier; resonant frequency, 600 Hz; Aurora Scientific; Aurora, Ontario ...

Cited by 45 - Related articles - Get at CISTI - All 5 versions

Altered single cell force-velocity and power properties in exercise-trained rat myocardium

[HTML] from physiology.org

GM Diffee… - Journal of Applied Physiology, 2003 - Am Physiological Soc

... The resulting skinned myocytes were resuspended in 8–10 ml of relaxing solution and kept on

ice throughout the days ... described previously (8). Skinned cardiac myocyte preparations were

attached between a capacitance-gauge transducer (model 403, Aurora Scientific) and a ...

Cited by 25 - Related articles - Get at CISTI - All 8 versions

Regional differences in effects of exercise training on contractile and biochemical properties of rat cardiac

myocytes

[HTML] from physiology.org

GM Diffee… - Journal of Applied Physiology, 2003 - Am Physiological Soc

... The resulting skinned myocytes were resuspended in 8–10 ml of relaxing solution and kept on

ice throughout the days ... described previously (9). Skinned cardiac myocyte preparations were

attached between a capacitance-gauge transducer (model 403, Aurora Scientific) and a ...

Cited by 24 - Related articles - Get at CISTI - All 5 versions

Force-length relations in isolated intact cardiomyocytes subjected to dynamic changes in mechanical load

[HTML] from physiology.org

G Iribe, M Helmes… - American Journal of Physiology- …, 2007 - Am Physiological Soc

... Gentaro Iribe, 1 Michiel Helmes, 1 ,2 and Peter Kohl 1. ... CCD, charge-coupled device. K was

measured directly, using a force transducer system (406A; Aurora Scientific, Aurora, ON, Canada)

factory calibrated for compression measurement, and mounted under a microscope. ...

Cited by 30 - Related articles - Get at CISTI - All 7 versions

Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells

[HTML] from rupress.org

…, N Harrell, CS Chung, M Helmes… - The Journal of …, 2011 - jgp.rupress.org

... Results were compared with those of previous studies on skinned myocytes (Helmes et al., 1996,

2003; Preetha et al., 2005) and ... fibers protruding from pipette tip), and their stiffness was determined

by cross-calibrating with a force transducer (model 406A; Aurora Scientific). ...

Cited by 5 - Related articles - All 6 versions

Calcium sensitivity and the Frank-Starling mechanism of the heart are increased in titin N2B region-deficient mice

[HTML] from nih.gov

EJ Lee, J Peng, M Radke, M Gotthardt… - Journal of molecular and …, 2010 - Elsevier

... papillary muscles were dissected into small strips (cross-sectional area (CSA) 0.02 mm 2 ; length

1.2 mm) and small aluminum clips were glued to the ends of the muscle [33] in order to attach

the muscle at one end to a force transducer (model 406, Aurora Scientific) and at the ...

Cited by 5 - Related articles - All 6 versions

Analysing force–pCa curves

[HTML] from nih.gov

JS Walker, X Li… - Journal of muscle research and cell motility, 2010 - Springer

... Department of Medicine, University of Colorado Denver, Aurora, CO 80045, USA e-mail:

john.s.walker@ucdenver.edu ... contrast ability at magnifications up to 400 9 . Sarcomere length

was measured for each myocyte using a image based system (Aurora Scientific 901A HSVL ...

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Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells

[HTML] from rupress.org

NMP King, M Methawasin, J Nedrud… - The Journal of …, 2011 - jgp.rupress.org

... Next, 10 ml of myocyte stopping buffer 2 was added (perfusion buffer plus 0.05 ml BCS/ml buffer

and 12.5 μM CaCl 2 ... mm of carbon fibers protruding from pipette tip), and their stiffness was

determined by cross-calibrating with a force transducer (model 406A; Aurora Scientific). ...

Cited by 5 - Related articles - All 6 versions

Inorganic phosphate speeds loaded shortening in rat skinned cardiac myocytes

[HTML] from physiology.org

AC Hinken… - American Journal of Physiology- …, 2004 - Am Physiological Soc

... were introduced at one end of the preparation with a direct current (DC) torque motor (model

308; Aurora Scientific) driven by ... window In a new window. Table 2. Effects of P i addition on

mechanical properties of skinned cardiac myocyte preparations. ... 2. Araujo A and Walker JW. ...

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Protein Kinase A–Mediated Acceleration of the Stretch Activation Response in Murine Skinned Myocardium Is

Eliminated by Ablation of cMyBP-C

[HTML] from ahajournals.org

JE Stelzer, JR Patel… - Circulation research, 2006 - Am Heart Assoc

... The ends of the preparation were attached to the arms of a motor (model 312B, Aurora Scientific)

and force transducer (model 403, Aurora Scientific), as described earlier. ... Circ Res. 1998; 83:

471–480. Abstract/FREE Full Text. ? de Tombe PP, Stienen GJM. ...

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Cycling cross-bridges increase myocardial stiffness at submaximal levels of Ca2+ activation

[HTML] from nih.gov

KS Campbell, JR Patel… - Biophysical journal, 2003 - Elsevier

... 913 ± 53 μm, width 130 ± 12 μm) were connected between a motor (Model 312B, time for

100-μm step 1 ms, Aurora Scientific, Ontario, Canada ... Many previous reports (Noble, 1977; Chiu

et al., 1982; de Tombe and ter Keurs, 1992; [Stuyvers et al., 1997] and [Stuyvers et al., 1998 ...

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Effect of stimulation rate, sarcomere length and Ca2+ on force generation by mouse cardiac muscle

[HTML] from physoc.org

BD Stuyvers, AD McCulloch, J Guo… - The Journal of …, 2002 - Physiological Soc

... The length of the muscle (ML) was set using a servo-controlled motor (model 318 B, Aurora

Scientific Inc., Aurora, ON, Canada) hooked to ... carbon fibre rod, constituted the force transducer

so that the resolution of the force measurement system was 0.5 μN (see Stuyvers et al. ...

Cited by 45 - Related articles - Get at CISTI - All 10 versions

Effects of thin and thick filament proteins on calcium binding and exchange with cardiac troponin C

[HTML] from nih.gov

JP Davis, C Norman, T Kobayashi, RJ Solaro… - Biophysical journal, 2007 - Elsevier

... Aluminum T-clips were used to mount the trabecula between the arms of a high-speed

length controller (model 322C, Aurora Scientific, Ontario, Canada) and an isometric force

transducer (model 403A, Aurora Scientific) in relaxing solution. ...

Cited by 39 - Related articles - Get at CISTI - All 8 versions

Fabrication and Evaluation of Reconstructed Cardiac Tissue and Its Application to Bio-actuated Microdevices

H Horiguchi, K Imagawa, T Hoshino… - NanoBioscience, …, 2009 - ieeexplore.ieee.org

... D. Contractile Force Measurement of the Reconstructed Cardiac Tissue The contractile force of

the reconstructed cardiac tissue was measured using a force transducer. One end of the tissue

was fixed to a permeabilized fiber test apparatus (803B, Aurora Page 3. ...

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The mechanoelectric feedback: a novel “calcium clamp” method, using tetanic contraction, for testing the role of

the intracellular free calcium

Y Yaniv, C Levy… - … of the New York Academy of …, 2006 - Wiley Online Library

... 308B, Aurora Scientific, Richmond Hill, Ontario, Canada) with a rise time of 250 μsec. ... from the

rat right ventricle (Spargue Dawley, 250–300 g). The dissection and the technique of mounting

the trabeculae in the experiment setup followed the procedures developed by ter Keurs. ...

Cited by 2 - Related articles - BL Direct - All 7 versions

Length dependence of force generation exhibit similarities between rat cardiac myocytes and letal muscle

fibres

[HTML] from physoc.org

LM Hanft… - The Journal of Physiology, 2010 - Wiley Online Library

... Length changes were introduced using a DC torque motor (model 308, Aurora Scientific, Inc.)

driven by voltage commands from a ... The kinetics of force redevelopment were obtained using

a procedure previously described for skinned cardiac myocyte preparations (Korte et al. ...

二、活体动物肌肉机械te性测试文献

Plasmid-based gene transfer in mouse letal muscle by electroporation

JD Schertzer, GS Lynch - METHODS IN MOLECULAR BIOLOGY- …, 2008 - Springer

... 1. The measurement of TA muscle function in situ requires a suitable platform (which is usually

Plexiglas) to secure the anesthetized mouse and for appropriate positioning of the servomotor. ...

Suitable systems are commer- cially available (Aurora Scientific, Aurora, ON, Canada). ...

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[HTML] < em> In Vivo</em> Canine Muscle Function Assay

[HTML] from jove.com

MK Childers, RW Grange… - Journal of Visualized …, 2011 - jove.com

... Stimulator, Grass, S88X, Stimulus isolation unit, Grass, SIU-C, Muscle lever system, Aurora

Scientific, 310C-LR, Footplate option. Signal interface, Aurora Scientific, Series 604A, Dynamic

Muscle Control & Analysis Software, Aurora Scientific, Aurora Scientific, ...

Cited by 1 - Related articles - Cached - All 4 versions

Monitoring Murine letal Muscle Function for Muscle Gene Therapy

[HTML] from nih.gov

CH Hakim, D Li, D Duan, CH Hakim, D Li… - Methods in molecular …, 2011 - Springer

... 1. Physiological saline (0.9% NaCl). 2. In situ muscle function assay system (Aurora

Scientific). The system consists of a 305C-LR dual mode transducer, a 701B stimulator,

a 604A signal interface, and an 809A footplate apparatus (Fig. 1c). ...

Androgen replacement therapy improves function in male rat muscles independently of hypertrophy and

activation of the Akt/mTOR pathway

C Hourde, C Jagerschmidt… - Acta …, 2009 - Wiley Online Library

... The right limbs were fixed with clamps (806A; Aurora Scientific, Aurora, Canada). The distal

tendons of the right soleus muscles were attached to a dual-mode lever arm system that measures

muscle isometric force (300C; Aurora Scientific) using a silk ligature. ...

Glutamine preserves letal muscle force during an inflammatory insult

BM Meador… - Muscle & nerve, 2009 - Wiley Online Library

... Force was measured using a dual-mode lever system, which controlled both length and

force/speed (305C-LR; Aurora Scientific). ... The ankle of the mouse was fixed in the servomotor's

footplate, with the talus (rotational center of the ankle joint) aligned with the servomotor axis. ...

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Sarcolipin overexpression in rat slow twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs

contractile function

[HTML] from jbc.org

AR Tupling, M Asahi… - Journal of Biological Chemistry, 2002 - ASBMB

... Three days after DNA injection, electrically evoked muscle force was assessed in situ from the

soleus muscle from each hindlimb across a range ... using a Grass S88 stimulator, and force data

were collected on-line using a 640A signal interface (Aurora Scientific Inc.) connected ...

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Tibialis anterior muscles in mdx mice are highly susceptible to contraction-induced injury

[PDF] from 141.213.232.243

C Dellorusso, RW Crawford, JS Chamberlain… - Journal of muscle …, 2001 - Springer

... no. 85-23 (NIH), Revised 1985, Office of Science and Health reports, Bethesda, MD). In

situ preparation ... The tendon of the muscle was tied securely to the lever arm of a servomotor

(Model 305B-LR, Aurora Scientific, Richmond Hill, ON, Canada). ...

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