biosearchtech,Amidites亚磷酰胺、BHQplus探针、双标记BHQ?探针、Dual-Labeled BHQ? Probes、BHQplus Probes

型号:DLO-RBP-2,DLO-RBP-5,DLO-QBP-1,DLO-QBP-2,DLO-QBP-5,DLO-GBP-2,DLO-GBP-1,
联系人:程园
联系电话:18611717737
品牌:biosearchtech

Biosearch Technologies是一家于创新性研发新型核酸产品以促进新基因的发现与应用的高科技公司。从上世纪八十年代开始,一代一代的Biosearch创新者就专注于寡核苷酸化学合成技术，在设计和生产荧光探针及引物中经过多次改良形成了公司te有的寡核苷酸修饰技术。目前 Biosearch开发的荧光粹灭技术Black Hole Quencher® (BHQ® dyes) 已成为高效荧光探针设计/合成的工业生产标准。以创新研发为原发动力的Biosearch Technologies公司已经通过英国ISO 9001:2008、ISO 13485:2003标准认证，获得加利福尼亚设备制造许可证以及GMP生产许可证。Biosearch拥有丰富的高品质产品线，不仅包括探针和引物产品，广泛应用于药物研发，DNA测序/SNP检测/基因表达分析等领域，也为其他寡核苷酸生产厂商提供原材料。公司在仪器生产领域的努力也使得我们能对公司产品的合成和纯化进一步深入和发展。

公司主要产品：

BHQplus Probes,BHQplus探针

Product Info

The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms.

BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”.

ValuMix? Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping.

Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available.

BHQplus probes feature:

• Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes
• High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch
• Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization
• A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign? software
• Bottom line: An economical alternative for probe-based SNP Genotyping

Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB?) probe and should first be analyzed for compatibility with the BHQplus chemistry.

NOTES:
Building Probes with Wobble/Degenerate Bases (What are Wobbles?)
To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.

Dual-Labeled BHQ® Probes双标记BHQ®探针

Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences.

We also offer ValuMix for gene expression and qPCR which delivers one BHQ probe and two primers in a single tube. Valumix primer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options.

Design Concerns for BHQ probes

Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB?) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact techsupport@biosearchtech.com with any questions regarding probe design.

NOTE:

·        Building Probes with an Internal Quencher (Currently available with FAM only)

·        Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification.

·        Building Probes with Wobble/Degenerate Bases (What are Wobbles?)

·        To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.

Best DEAL:

·        $98 ValuProbe? BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days 

Instrument CALIBRATION:

·        FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase

Learn MORE:

·        Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays

·        The difference between FRET and static quenching

Assay DESIGN:

·        Try RealTimeDesign? Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!