型号:
联系人:李胜亮
联系电话:18618101725
品牌:匈牙利cellsorter
该系统是一种安装在正常倒置显微镜上的计算机控制的微量吸管系统,量化细胞形变、细胞间粘附作用和细胞与基质之间的粘附作用,可同步实现荧光观测、单细胞捕获、分选和分离和图像视频处理
一个可在已有显微镜上搭建的,可同步实验吸吮加载、图像视频处理与荧光观测的细胞力学装置。可建立微管吸吮力学加载-荧光观测耦合的分子-细胞动力学实时原位观测系统。
单个细胞粘附力测定模式图:
细胞与细胞之间粘附力测定模式图:
通过施加负压将细胞的一部分(或整体)吸入微管中,测量一定负压吸吮作用下细胞的变形及其时间历程或细胞粘附分离的临界负压来评价细胞的变形特性,同时记录细胞的吸入量。
图像处理技术和力学模型,量化测量细胞形变、细胞对之间的相互作用以及黏附特性、基质附着细胞测定细胞硬度等力学特性。
压电微管吸吮模块:对样品池内细胞的捕获、分选、吸吮和微操控;
集成倒置荧光相差显微镜:用于对所述细胞进行荧光激发;
信号采集模块:用于对微弱荧光信号的采集;
控制模块:用于对微管吸吮和荧光采集同步触发,并进行数据分析处理
1)High throughput single cell sorting directly from the Petri dish
One single cell arrives to each PCR tube
10 PCR strips containing 80 tubes can be filled in a cycle
Glass cover slip for testing single cell deposition in situ
Drop volume less than 1 ul for adherent cells
Pick up volume of ~1 nl for suspended cells
15-20 seconds per cell. When collecting multiple cells, sorting speed is 1 cell/second.
Number of cells picked up in a single run: 1-1000.
Isolates a subpopulation of live adherent cells expressing fluorescent or luminescent markers
Both unlabeled and fluorescentcells are recognized by computer vision
Viable cells after sorting
Any adherent and non-adherent cell type can be sorted
Cell culture needs minimal preparation before sorting
Average sorting process takes only a few minutes
Multichannel detection using the fluorescent filter setup of the microscope
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