Dot-Cult,Confo-Cult,Porous Microcarrier PMc（可生物降解的微载体）,ECM Graft,Non-Adherent Culture Dish,非粘附培养皿(干细胞和微球非粘附培养皿)，共聚焦显微镜3D细胞培养皿

型号:Dot-Cult,Confo-Cult,Porous Microcarrier PMc,ECM Graft,Non-Adherent Culture Dish
联系人:李胜亮
联系电话:18618101725
品牌:excell matrix

Dot-Cult®

A Convenient 3D Cell Culture Method

Dot-Cult^® is designed to perform 3D cell culture without compromising convenience of monolayer culture (2D cell culture) in a native ECM (extracellular matrix) like microenvironment.

All that is required for Dot-Cult^® is to add media and cells to setup 3D culture.

Principles of Dot-Cult^® can be adapted to any cell culture formats like multiwell-plate, flask, hanging drop culture, slide culture etc.

Researchers not only can perform 3D cell culture conveniently but also evaluate cell attachment, growth using normal microscopy procedures. Cells can be quantified under 3D culture using Dot-Cult^® and harvested by normal enzymatic method.

As an enthusiastic customer has expressed “it is fastest way to get a publication”. We guarantee an innovative touch to your research.

Please discuss with our experts your unique cell culture requirements and explore options beyond conventional products.

Product Design Concept

Three dimensional (3D) Cell culture of human and animal cells mimic natural tissue interactions better than 2D monolayer culture for any practical purpose.

Matrigel, collagen and reconstituted extracellular matrix as hydrogels are popular among cell biologists to create tissue model due to their ability to mimic extracellular micro-environment. Porous scaffolds and hydrogels of biodegradable polymers are preferred for tissue engineering research due to robust handling properties.

Finding a common biomaterial that has both ECM mimicry and engineering properties has been difficult. Further, delivering the biomaterial as cost effective ready-to-use devices has not been achieved.

Dot-Cult^® is designed to provide 3D cell culture in popular and convenient Petri dish and multiwell plate formats.

3D culture is achieved in specified ECM Analog^® scaffold coated area (a dot) of Dot-Cult^®instead of entire surface of Petri dish. Uncoated area of Petri dish is treated for non-adhesion of cells to prevent any 2D-monolayer growth of cells. ECM Analog^® coated area is kept optimal to provide proportionate number of cells to the culture volume.

Consequently, Dot-Cult^® for 3D cell culture remains identical to a Petri dish in terms of convenience, acquaintance, no prior training or exposure, medium requirement, duration of culture, trypsinization and handling robustness.

Highlights

1. Dot-Cult^® is gamma irradiated and available as ready to use package.
2. 3D dots are available in two types
   1. Collagen Type I
   2. Amniotic ECM (coming soon)
3. Also available with 2D control dot for easy comparison.
4. Customizable Extra Cellular Matrix for 3D culture of specific cell types.
5. Customizable scaffold features (pore size, particle size etc.).
6. Just add cells, media and incubate.
7. Change media as per monolayer culture.
8. Harvest cells by usual trypsinization.
9. Long term 3D cell culture possible.
10. Multiple cell type can be inoculated in single plate to create organ like features.

Dot-Cult^® is for research purpose only.

Confo-Cult®

3D Cell Culture for Confocal Microscopy

Confo-Cult^® is designed to study differentiation/ cell functionality under 3D Cell Culture using fluorescence and confocal microscopic techniques.

3D cell culture is performed on a slide that can be retrieved (unlike Dot-Cult^®) from culture dish and mounted on microscope.

Researchers not only can perform 3D cell culture using Confo-Cult^® but also evaluate the performance using fluorescence and confocal microscopy for differentiation, proliferation etc. such integrated performance is not offered by any technology as yet.

As an enthusiastic customer has expressed “it is fastest way to get a publication”. We guarantee an innovative touch to your research.

Please discuss with our experts your unique cell culture requirements and explore options beyond conventional products.

Product Design Concept

Physiological relevance of three dimensional (3D) cell cultures has made it a fundamental research tool in cell biology. 3D cell cultures are used in a broad range of cell biology research, including tumor biology, cell adhesion, cell migration, metastasis, angiogenesis and epithelial morphogenesis. Hydrogels like matrigel and collagen are popular among cell biologists to create 3D tissue model due to their ability to mimic extracellular micro-environment. Porous scaffolds of biodegradable polymers on the other hand, are preferred for tissue engineering research for 3D cell cultures due to robust mechanical properties.  3D cultures expected to decrease laboratory animals in drug screening and toxicity assays.

Analysis of 3D cell culture requires delicate handling of 3D hydrogel from culture plates that may disturb the cellular arrangements. 3D cell culture samples are highly scattering due to thickness (few hundred microns) which is a challenge for microscopic analysis.

Researchers very often prefer to grow cells on cover slips as monolayers for confocal microscopy studies missing the advantages of 3D cell culture. Innovations both in 3D cell culture formats and analysis techniques are essential to exploit the benefits of third dimension in the life sciences. 3D cell culture for advanced microscopy has been made easy and cost effective with Confo-Cult^®. ECM Analog^® coating of porous scaffold on typical cover slips makes it not only easier to perform 3D cultures but also simplifies confocal microscopic observations and high content analysis.

Highlights

1. Confo-Cult^® is gamma irradiated and available in ready to use packing.
2. 3D dots are available in two types of ECM material.
   1. Collagen Type I
   2. Amniotic ECM (coming soon)
3. Also available with 2D dot for easy comparison.
4. Confo-Cult^® is designed for 3D cell culture for Confocal Microscopy.
5. Customizable Extra Cellular Matrix for 3D culture of specific cell types.
6. Customizable scaffold features (pore size, particle size etc.).
7. Handling is identical to monolayer culture.
8. Cells do not grow on scaffold free area.
9. Just add cells, media, and incubate.
10. Multiple cell types can be co-cultured to create organ like features.

Non-Adherent Culture Dish

For Stem Cell and Spheroid Culture

Non-Adherent Culture Dish are used for stem cell and spheroid culture. Special hydrophilic type coating on Petri dish makes it non-adherent to proteins. Compared to three-dimensional, histiotypic cultures, cells grown as monolayers may be more affected by the culture conditions, due to reduced cell-to-cell interactions. Multi-cellular spheroids formed by cells are widely used as avascular tumor models for metastasis and invasion research and for therapeutic screening.

Customizable culture formats

1. Multi-well plates (6 well, 12 well, 24 well etc.)
2. Large dishes
3. Tissue Culture flasks

Non-Adherent Culture Dish is for research purpose only.