细胞体积和压力调控机制调控系统-细胞体积厚度等动态限制器和静态限制器

型号: cell confiner
联系人:李胜亮
联系电话:18618101725
品牌:法国




CELL CONFINEMENT

Confine your cells and control their 3D shape

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    Define the thickness or 3D shape of plated cells

    Control the thickness of your cells with the right confiner slide.

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    Image your cells in a convenient fashion

    With the cell confiner you see all the cell organelles in the same plane, removing optical aberrations and simplifying the imaging process.

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    Control over the cell microenvironment

    Choose the environment features: geometric adhesion, environment elasticity, surface chemistry…

DYNAMIC CONFINER

A step towards mimicking the natural cell environment

  • Control the confinement speed

    Accurate control of the confinement speed through the vacuum pump

  • Reversible confinement: retrieve your cells after confinement

    Allows molecular analysis thanks to the non-destructive method for the cells

  • Compatible with your own experiments

    The confiner is a small size device that is directly placed on the top of your cell culture

The dynamic cell confiner is a device that allows confining cells within two surfaces with micrometer precision. The space between the two surfaces is controlled by micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston (suction cup). The piston is controlled with a vacuum pump, and the height of the confinement is thus controlled as well. The device can be used in a petri dish, in a glass substrate, etc.

2、6-Wells Static Confiner (CO2 plug)

EXPLORE CELL MECHANICS

6-WELLS STATIC CONFINER WITH CO2 PLUG – CSOW 620

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  • Define the thickness and shape of cells 

    Control the thickness of your cells with the right confinement slide

  • Multiple experiments simultaneously

    Enables the study of different cells or to apply different confinement conditions at the same time

  • Adaptable with high resolution microscopy

    Optically transparent materials and compact design enable high resolution microscopy

The CSOW 620 – static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston. The CSOW 620 is the device that controls the position of the pistons and it is compatible with several brands of 6-well plates.  In the end of the confinement experiments, the cells can be retrieved enabling further biochemical studies. The user decides when and where to confine the cells as it is not necessary to plug it to any other device. In particular, the CSOW 620 is characterized by its compact design and CO2 plug.

Overall dimensions of the CSOW 620:

CSOW620_1

Scheme of the CSOW 620:

CSOW620_2
CSOW620_3

The static cell confiner CSOW 620 kit is composed by:

  • 1 x CSOW 620
  • 12 x Confinement Piston in PDMS
  • 12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)

If you want to make your slides adhesive to your cells or not (optional):

  • Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
  • Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

 

If this device does not fit with your constraints, get in touch with us! We can personalize it for you!


3、1-Well Static Confiner

DYNAMIC CONFINER

A step towards mimicking the natural cell environment

  • Control the confinement speed

    Accurate control of the confinement speed through the vacuum pump

  • Reversible confinement: retrieve your cells after confinement

    Allows molecular analysis thanks to the non-destructive method for the cells

  • Compatible with your own experiments

    The confiner is a small size device that is directly placed on the top of your cell culture

The dynamic cell confiner is a device that allows confining cells within two surfaces with micrometer precision. The space between the two surfaces is controlled by micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston (suction cup). The piston is controlled with a vacuum pump, and the height of the confinement is thus controlled as well. The device can be used in a petri dish, in a glass substrate, etc.

The dynamic cell confiner kit is composed by:

  • 1 x Elveflow Vacuum & Pressure Generator and 1 x Elveflow AF1 DUAL – Vacuum & Pressure Controller
  • 10 x Confinement Slides – 10 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement.
    Available confinement heights – from 1, 2, 3, … up to 20 um (choose up to 3 heights to integrate your kit)
  •  3 x Dynamic Confiner? (PDMS Suction Cup)
  •  1 x security tank (to preserve the vacuum pump)
  •  1 x Kit of Tubes and Connectors

If you want to make your slides adhesive to your cells or not (optional):

  • Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
  • Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

4、6-Wells Static Confiner


EXPLORE CELL MECHANICS

6-WELLS STATIC CONFINER WITH CO2 PLUG – CSOW 620

test15
  • Define the thickness and shape of cells 

    Control the thickness of your cells with the right confinement slide

  • Multiple experiments simultaneously

    Enables the study of different cells or to apply different confinement conditions at the same time

  • Adaptable with high resolution microscopy

    Optically transparent materials and compact design enable high resolution microscopy

The CSOW 620 – static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston. The CSOW 620 is the device that controls the position of the pistons and it is compatible with several brands of 6-well plates.  In the end of the confinement experiments, the cells can be retrieved enabling further biochemical studies. The user decides when and where to confine the cells as it is not necessary to plug it to any other device. In particular, the CSOW 620 is characterized by its compact design and CO2 plug.

Overall dimensions of the CSOW 620:

CSOW620_1

Scheme of the CSOW 620:

CSOW620_2
CSOW620_3

The static cell confiner CSOW 620 kit is composed by:

  • 1 x CSOW 620
  • 12 x Confinement Piston in PDMS
  • 12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)

If you want to make your slides adhesive to your cells or not (optional):

  • Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
  • Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

 

If this device does not fit with your constraints, get in touch with us! We can personalize it for you!


什么是细胞限制器?

Cell Confiner再现了细胞通常生活在体内的条件:它们受到限制。它允许您控制细胞的厚度和细胞培养的体积。通过非破坏性方法,您可以j确定义培养物的体积,以及施加在细胞上的te定压力。这构成了一种新的相关系统,用于培养引发生物仿生学的细胞。

我怎么用呢?

限制器与标准板(例如petridish / fluorodish或6孔板)兼容。它还兼容高分辨率实时成像和标准生物分析。

动态和静态限制器之间有什么区别?

静态限制器提供了在静态下观察限制对细胞的影响的可能性,并将其与“正常”细胞状态进行比较。
动态限制器适用于以动态方式改变细胞上的限制,限制和检索甚至改变强度。

该设备可以使用多少次?

Cell Confiner可以多次使用,具体取决于使用和处理。为了保持更长时间,请务必轻轻地处理它们,并在每次使用时使用70%乙醇正确清洁它们。
每个系统都包含需要更改的内容,以便您在需要时至少更换一些部件。我们还根据需要提供额外的消耗品。

怎样才能控制培养物的高度?

由于固定在载玻片上的微柱系统可以控制培养物的高度,这些系统由吸盘推/拉。支柱的高度可以从3μm调节到20μm。