High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transon.
Cytokine-induced molecular responses in airway smooth muscle cells inform genome-wide association studies of asthma.
References
Vogel, V. & Sheetz, M. Local force and geometry sensing regulate cell functions. Nat Rev Mol Cell Biol 7, 265-275 (2006).
Wang, N., Tytell, J. D. & Ingber, D. E. Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus. Nat Rev Mol Cell Biol 10, 75-82, doi:nrm2594 [pii] 10.1038/nrm2594 (2009).
Davies, P. F., Spaan, J. A. & Krams, R. Shear stress biology of the endothelium. Ann Biomed Eng 33, 1714-1718 (2005).
Hahn, C. & Schwartz, M. A. Mechanotransduction in vascular physiology and atherogenesis. Nat Rev Mol Cell Biol 10, 53-62, doi:nrm2596 [pii] 10.1038/nrm2596 (2009).
Akilesh, S. et al. Arhgap24 inactivates Rac1 in mouse podocytes, and a mutant form is associated with familial focal segmental glomerulosclerosis. J Clin Invest 121, 4127-4137, doi:10.1172/JCI46458 (2011).
Sanz-Moreno, V. et al. Rac activation and inactivation control plasticity of tumor cell movement. Cell 135, 510-523, doi:S0092-8674(08)01236-1 [pii] 10.1016/j.cell.2008.09.043 (2008).
Saito, K., Ozawa, Y., Hibino, K. & Ohta, Y. FilGAP, a Rho/ROCK-regulated GAP for Rac controls tumor cell migration. Mol Biol Cell, doi:mbc.E12-04-0310 [pii] 10.1091/mbc.E12-04-0310 (2012).
Ehrlicher, A. J., Nakamura, F., Hartwig, J. H., Weitz, D. A. & Stossel, T. P. Mechanical strain in actin networks regulates FilGAP and integrin binding to filamin A. Nature (2011).
Yoshie H, Koushki N, Kaviani R, Tabatabaei SM, Rajendran K, Dang Q, Husain A, Yao S, Li C, Sullivan J, Saint-Geniez M, Krishnan R*, Ehrlicher AJ*. Traction force screening enabled by compliant PDMS elastomers. Biophys J, 114(9): 2194-2199, 2018. * contributed equally
Yoshie H, Koushki N, Molter C, Siegel PM, Krishnan R, Ehrlicher AJ. High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transon. J Vis Exp. Jun 1;(148), 2019.
