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水凝支架三维培养系统，水凝胶基质力学环境模拟系统，水凝胶基质力学环境系统，生物材料细胞力学微环境体外构建系统

型号:fx-5000tt
联系人:李胜亮
联系电话:18618101725
品牌:美国flexercell

三维种子细胞构建人工生物组织系统(Creating a Bioartifical Construct with the Tissue Train System)

特点：

1）对生长在三维状态下的细胞进行静态的或者周期性的牵张拉伸刺激培养，可以进行实时观察分析。

2）对生长在三维环境下的细胞进行单轴向或者双轴向的静态或者周期性的应力加载实验

3)可建立te制的各种模拟实验：心率模拟实验，步行模拟实验，跑动模拟实验和其他动力模拟实验。
4)可构建长度达35mm的生物人工组织

5）具有丰富的三维培养模具和多种蛋白包被材料的自动细胞组织三维培养系统

6）该系统以立体水凝胶为三维培养支架，水凝胶支架具有大量体内微环境基质的特征，水凝胶所具有的三维网络结构、含水量高和力学性能可控等特性与体内细胞所处基质微环境相似, 被广泛用于工程化组织的体外构建研究，水凝胶的硬度调控范围很大, 非常有利于模拟体内生理或病理力学微环境
是真正意义上的三维培养系统

7）配套的scanflex扫描分析模块可以记录三维人工组织中凝胶的压实过程、记录三维细胞培养凝胶的压实动力学、凝胶面积计算

An automated scanning device with area measurement software.

Measure gel compaction in 3D bioartificial tissues.
Scans and saves images up to 600 dpi of 3D tissue constructs.
Can be used in conjunction with the Tissue Train^® Culture System.

适用范围

1）flexercell的Tissue Train ®培养体系，是为了解决这一组织培养过程中的难题，这个培养体系通过为细胞和基质提供三维支架矩阵组织、动态的拉伸力和多种几何模型来创建不同形状的生物人工组织（如线性，梯形和圆形）。

2水凝胶基质力学环境模拟

3）生物材料的细胞力学微环境体外构建系统

4）基于干细胞3D力学环境的工程化微组织构建研究

Tissue Train^® Bioartificial Tissue Fabrication with Uniaxial Strain

A 3D collagen cell-seeded construct (or bioartifical tissue) is dispensed with a pipette into a linear mold created with the Trough Loader® and Tissue Train^® System. After the construct has polymerized, the flexercell^® Tension System can be used with an Arctangle^® Loading Station? to apply uniaxial strain to the construct.

Tension Test of a Bioartificial Tissue
A 3D cell-seeded collagen gel created with the Tissue Train^® System is subjected to a tensile test until failure. Shown here is the construct within the test grips during testing

Tissue Train^® Trapezoidal Construct under Tension with Corresponding Finite Element Strain Values
Trapezoidal-shaped 3D cell-seeded gel construct (created with the flexercell^® Tissue Train^®System) undergoing unconstrained tension applied with the FX-5000? Tension System. The strain values, as determined with Finite Element Analysis, are depicted alongside the strained construct.

Tissue Train ®培养系统应用背景

体外培养在与真实组织在结构上和功能上相似的人工组织需要以下几个基本条件：

（1）细胞

（2）支架矩阵组织

（3）培养基和生长因子和（4）机械刺激。这些条件彼此相互影响，并且相互之间共同来促进形成能够承受生物机械力的，且结构比较稳定的组织。而在人工组成形成的过程中，这些细胞按照发育途径形成具有一定几何形状的细胞外基质结构。其中一些信号转导途径参与了细胞外基质组合物的形成。这些途径中，有些是由细胞基质的机械变形调节，并通过膜结合蛋白，如整合素，粘着斑复合体，细胞粘附分子和离子通道传递到细胞内。这些途径中细胞还可以响应配体，如细胞基质形变所释放的细胞因子，激素或生长因子等。

为了维持肌肉骨骼组织的完整性和强度，组织内细胞需要保持一定水平的的内在应力。如果缺乏这种内在的应力，组织会缺少强度导致细胞结构的破坏或者组织的断裂。目前一般认为如果在固定四肢，卧床休息或在内在应力水平的降低的情况下，将导致骨中矿物质流失，骨组织萎缩，骨骼弱化，以及合成代谢活性的降低和分解代谢活性的增加。

为了在体外培养与原生组织类似的人工组织，重要的就是能够创建模拟体内条件的环境。细胞在具有机械运动作用的的环境中培养，可以促进细胞的新陈代谢，并可以改变细胞的形状和其它性能。因此，在体外形成过程中建立和保持一个具备机械作用的环境（即张力，剪切力或压缩）就成为这一过程中至关重要的。除了具备机械作用的环境，在三维环境下培养细胞可以比静态二维培养法更好地模拟原生环境。

典型应用文献摘选：

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6. Cao TV, Hicks MR, Campbell D, Standley PR. Dosed myofascial release in three-dimensional bioengineered tendons: effects on human fibroblast hyperplasia, hypertrophy, and cytokine secretion. J Manipulative Physiol Ther 36(8):513-21, 2013.

7. Cao TV, Hicks MR, Zein-Hammoud M, Standley PR. Duration and magnitude of myofascial release in 3-dimensional bioengineered tendons: effects on wound healing. J Am Osteopath Assoc 115(2):72-82, 2015.

8. Charoenpanich A, Wall ME, Tucker CJ, Andrews DM, Lalush DS, Loboa EG. Microarray analysis of human adipose-derived stem cells in three-dimensional collagen culture: osteogenesis inhibits bone morphogenic protein and Wnt signaling pathways, and cyclic tensile strain causes upregulation of proinflammatory cytokine regulators and angiogenic factors. Tissue Eng Part A 17(21-22):2615-2627, 2011.

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14. de Lange WJ, Grimes AC, Hegge LF, Ralphe JC. Ablation of cardiac myosin-binding protein-C accelerates contractile kinetics in engineered cardiac tissue. J Gen Physiol 141(1):73-84, 2013.

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18. Henshaw DR, Attia E, Bhargava M, Hannafin JA. Canine ACL fibroblast integrin expression and cell alignment in response to cyclic tensile strain in three-dimensional collagen gels. J Orthop Res 24(3):481-490, 2006.

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21. Jones ER, Jones GC, Legerlotz K, Riley GP. Cyclical strain modulates metalloprotease and matrix gene expression in human tenocytes via activation of TGFβ. Biochim Biophys Acta 1833(12):2596-2607, 2013.

22. Lee CH, Shin HJ, Cho IH, Kang YM, Kim IA, Park KD, Shin JW. Nanofiber alignment and direction of mechanical strain affect the ECM production of human ACL fibroblast. Biomaterials 26(11):1261-1270, 2005.

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24. Nguyen MD, Tinney JP, Ye F, Elnakib AA, Yuan F, El-Baz A, Sethu P, Keller BB, Giridharan GA. Effects of physiologic mechanical stimulation on embryonic chick cardiomyocytes using a microfluidic cardiac cell culture model. Anal Chem 87(4):2107-13, 2015.

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26. Nourse MB, Halpin DE, Scatena M, Mortisen DJ, Tulloch NL, Hauch KD, Torok-Storb B, Ratner BD, Pabon L, Murry CE. VEGF induces differentiation of functional endothelium from human embryonic stem cells: implications for tissue engineering. Arterioscler Thromb Vasc Biol 30(1):80-89, 2010.

27. Peters AS, Brunner G, Krieg T, Eckes B. Cyclic mechanical strain induces TGFβ1-signalling in dermal fibroblasts embedded in a 3D collagen lattice. Arch Dermatol Res 307(2):191-7, 2015.

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45. Weinbaum JS, Schmidt JB, Tranquillo RT. Combating adaptation to cyclic stretching by prolonging activation of extracellular signal-regulated kinase. Cellular and Molecular Bioengineering 6(3):279-286, 2013.

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